Cerebellar nuclei neurons show only small excitatory responses to optogenetic olivary stimulation in transgenic mice: in vivo and in vitro studies

To study the olivary input to the cerebellar nuclei (CN) we used optogenetic stimulation in transgenic mice expressing channelrhodopsin-2 (ChR2) in olivary neurons.We obtained Saw Blades in vivo extracellular Purkinje cell (PC) and CN recordings in anesthetized mice while stimulating the contralateral inferior olive (IO) with a blue laser (single p

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Increased interactions and engulfment of dendrites by microglia precede Purkinje cell degeneration in a mouse model of Niemann Pick Type-C

Abstract Niemann Hand Mixer Pick Type-C disease (NPC) is an inherited lysosomal storage disease (LSD) caused by pathogenic variants in the Npc1 or Npc2 genes that lead to the accumulation of cholesterol and lipids in lysosomes.NPC1 deficiency causes neurodegeneration, dementia and early death.Cerebellar Purkinje cells (PCs) are particularly hyperse

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